Genome Editing

TILLING shows its limitations when targeting genes belonging to multigenic families. Methods for modifying multiple genes simultaneously, based on CRISPR / Cas9 genomic editing technology, offer new solutions to this problem. For this we have established MultiCrisp pipline. The objective of this pipline is to facilitate the functional analysis of redundant gene families in plants and to create agronomic traits of interest.

CRISPR–APOBEC1 Extensive Allele Mutagenesis and TILLING-mediated variability Enrichment (CREATE)