SUCCESS OF THE FIRST TRANSPARIZATIONS OF WHOLE PLANT ORGANS IN FRANCE WITH THE X-CLARITY SYSTEM from Logos Biosystems

IPS2 has recently been equipped with an X-Clarity transparency station (Logos Biosystems), funded by the University of Evry Val d'Essonne. This system has been implemented on the EPITRANS platform. For the first time in France, the totally transparency of plant organs, such as melon flowers, has been achieved with this equipment. A 3D reconstitution of samples, through acquisitions with the Light Sheet Fluorescence Microscope (LSFM Z1 Zeiss, IDEX Paris-Saclay) available at IPS2 is then possible. This type of sample preparation, in addition to optimizing the use of the LSFM by avoiding microtome sections, reduces photo-toxicity and photo bleaching.

The implementation of this new protocol on the EPITRANS platform will make it possible to combine it with the use of the LSFM, thus making it possible to overcome tissue opacity and to visualize the localization of the expression of several genes of interest by using fluorescent reporters or labeled microorganisms interacting with this organ.

The EPITRANS platform is thus developing its cell imaging analysis capacity for the functional validation of genes of agronomic interest in crops.

List of plant species and whole organs tested on the X-Clarity transparency system on the EPITRANS platform:

Melon (flowers), peas (leaves), wheat (embryos, leaves, spikelets), Arabidopsis (siliques, stems), Medicago (nodules), tomato (flowers), tobacco (leaves), bean (leaves)

Contact: Halima.Morin@inrae.fr

Quelques images pour illustrer :

Silique entière d’Arabidopsis thaliana

Fleur entière de melon

Feuille de haricot